Regulatory
KB2

Part:BBa_K3777003

Designed by: SiYuan Peng   Group: iGEM21_OUC-China   (2021-10-01)


kleptamers

an antisense RNA strand which is partially complementary to 3WJdB(BBa_K3777000)

800px-T--OUC-China--result--V.png
Verifying the strand replacement reaction. Promoter J23104 is much stronger than J23110. TetR-T7(tetO)-3WJdB has no KB2, so it is set as a negative control.
The result of this experiment proved that it’s valid to repress the fluorescence in vivo, which strongly support the feasibility of the ‘improved circuit’.

Reference：Alam Khalid K,Tawiah Kwaku D,Lichte Matthew F,Porciani David,Burke Donald H. A Fluorescent Split Aptamer for Visualizing RNA-RNA Assembly In Vivo.[J]. ACS synthetic biology,2017,6(9)
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None